The Australian was defaulted from his second-round match against Casper Ruud at the Italian Open with the scores poised at one set apiece.
Kyrgios was handed a one-game penalty for unsportsmanlike conduct when he appeared to shout at a member of the crowd after his serve was broken in the final set.
He then threw his racket on the floor, kicked over a water bottle, and launched a chair into the middle of the court before saying “I’m done.” According to ATP regulations, a defaulted player loses all prize money, hotel accommodation and points accrued during that event.
The result also means that Ruud, who had been leading 2-1 in the final set, will face Juan Martin del Potro in the next round.
These events come shortly after Kyrgios, ranked No. 36 in the world, had taken aim at rivals Novak Djokovic and Rafael Nadal.
“This whole celebration thing that he does after matches, it’s like so cringeworthy … No matter how many grand slams he wins, he will never be the greatest for me.”
Kyrgios, who won the fifth ATP title of his career in Mexico earlier this year, had overcome Russian Daniil Medvedev 6-3 3-6 6-3 in the first round of matches in Rome.
The 24-year-old is no stranger to controversy.
Kyrgios took to Twitter following Thursday’s game at the Italian Open, laughing in response to a video of Ruud celebrating after the match.